PURIFICATION OF THE INTERSTITIAL CELL-STIMULATING AND FOLLICLE-STIMULATING HORMONES OF THE PITUITARY
نویسندگان
چکیده
منابع مشابه
[Purification of follicle-stimulating hormone from human pituitary glands].
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملPurification of Follicle-stimulating Hormone from Human Pituitary Glands*
A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...
متن کاملPhysicochemical Characterization of Pituitary Follicle-stimulating Hormone
The physiochemical characteristics of the follicle-stimulating hormone (FSH) from whole sheep pituitary glands have been studied. The hormone behaves as a single protein in electrophoresis, diffusion, and ultracentrifugation. It has an isoelectric point at pH 4.5 and a molecular weight of 67,000 and contains 1.23 per cent hexose and 1.51 per cent hexosamine. The amino acid composition has also ...
متن کاملPurification and properties of interstitial cell-stimulating hormone from sheep pituitary glands.
Early investigations on the purification and characterization of the hypophyseal interstitial cell-stimulating hormone yielded highly active products from sheep (l-3) and swine (4,5) pituitary glands. The preparations appeared to be homogeneous according to the physical and biological criteria of purity then available. The importance of the early ovine and porcine preparations is attested by th...
متن کاملDevelopmental Patterns of Follicle-stimulating, Luteinizing and Thyroid-stimulating Hormones in the Hypothyroid Female Rat
The developmental patterns of plasma and pituitary levels of the three glycoprotein hormones, FSH, LH and TSH, were studied in hypothyroidism and after thyroxine administration. Rats were radiothyroidectomized at birth and the pituitary and circulating hormone levels were measured at five-day intervals from 5 to 4o days of age by double-antibody radioimmunoassay. Body weight and weight of selec...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1940
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)73142-6